摘要
目的 为实验室日常检测近交系小鼠的遗传背景提供一种分子生物学方法。方法 用 6条随机引物对 6个品系近交系小鼠基因组DNA进行PCR扩增。结果 6条随机引物中p2、p3、p5和p6四引物扩增的条带差异较为明显。
Objective To study a new method for inbred strain mice genetic monitoring.Method 6 random primers were used to amplify DNA in 6 inbred strains of mice (TA1, TA2, T739, C57BL/6,615 and Scid). Results The amplified band patterns were obviously different in the products of 4 primers(p2,p3,p5 and p6).Conclusion The results indicated that different strains of mice could be easily distinguished by RAPD method.
出处
《中国实验动物学报》
CAS
CSCD
2003年第3期184-186,共3页
Acta Laboratorium Animalis Scientia Sinica
