摘要
目的比较长爪沙鼠与实验大、小鼠的RAPD图谱,为实验室日常鉴别动物品种品系提供一种分子生物学方法。方法提取基因组DNA(封闭群沙鼠、近交系小鼠BALB/c和C57BL/6、封闭群小鼠KM、近交系大鼠F344和BN以及封闭群大鼠SD),用6条随机引物对其进行PCR扩增。结果在6条随机引物中,p1、p2、p3、p4和p6这5条引物扩增的条带差异较为明显,表现为不同的RAPD图谱。结论RAPD方法可用于鉴定长爪沙鼠与实验大小鼠的差异。
Objective A comparison of RAPD maps of gerbils and laboratory rats and mice was made in order to explore a new method for distinguishing different strains of laboratory animals. Methods DNA was extracted from the tail tissues from different strains of animals （gerbil, BALB/c, C57BL/6, KM, F344, BN, SD）. Six pairs of primers were used to amplify their DNA. Results The amplified band patterns were apparently different in the products of 5 primers （p1, p2, p3, P4 and p6）. Conclusion Our results indicate that it is feasible to distinguish different strains of laboratory animals by RAPD method.
出处
《中国比较医学杂志》
CAS
2006年第6期353-355,共3页
Chinese Journal of Comparative Medicine
