摘要
目的建立以石油醚洗脱杂质测定巴西绿蜂胶胶囊中总黄酮的方法。方法样品经乙醇提取,用聚酰胺粉吸附,分别用石油醚、苯不同溶剂洗去杂质;经甲醇洗脱后,在波长360 nm处,用分光光度法测定巴西绿蜂胶中总黄酮的含量。结果采用石油醚作为洗脱剂,总黄酮含量在1.9897~23.8761μg/m L范围内,与吸光度值呈良好的线性关系,浓度-吸光度回归方程为Y=0.02976X-0.00741,相关系数r2=0.99954。方法精密度为1.0%,平均回收率为96.5%。与用苯洗脱杂质结果比较,两者无显著性差异。结论该方法操作简便,重现性及稳定性好,线性范围宽,减少了苯对检测人员和环境的污染,可用于保健食品中总黄酮的测定。
Objective To establish a method for determining flavonoids in Brazil green propolis capsule. Methods The total flavonoids in Brazil green propolis capsule were determined by spectrophotometry after the samples were distilled by methanol and purified by petroleum ether. The detection wavelength was 360 nm. Results The calibration curve of flavonoids content and absorbance had a good linearity in the range of 1.9897~23.8761 μg/mL (r2=99954). The relative standard deviations were 1.0%, and the recoveries were 96.5%. The results had no obvious difference which purified by petroleum ether instead of benzene. Conclusion The method is simple, stable and accurate, and it can be applied successfully to determine flavonoids in health food.
出处
《食品安全质量检测学报》
CAS
2015年第8期3060-3063,共4页
Journal of Food Safety and Quality
