摘要
目的 探讨重组人生长激素 (rhGH)对肝硬化大鼠在肝功能、门静脉高压等方面的治疗作用及其可能的机制。方法 雄性SD大鼠建立肝硬化模型 ,随机分组 ,分别给予NS、rhGH (333ng/KgBW ,ip ,qd× 7d)处理。 结果 rhGH处理前后肝硬化肝组织GHR的配体结合容量 [fmol/mg ,(31± 4 )vs .(4 0± 7) ]及其mRNA量 (iOD ,pixel)均显著升高 (2 3± 3)vs.(4 2± 8) ;P <0 0 5 ,肝硬化大鼠血清白蛋白 (g/L )显著升高 (2 9± 4 )vs .(37± 7) ;P <0 0 5、ALT活性 (U/L)显著降低 (89± 15 ,6 9± 7;P <0 0 5 ) ,肝硬化肝组织丙二醛含量 (nmol/mg)显著降低 (18 7± 3 2 ,12 0± 2 2 ;P <0 0 5 )、超氧化物歧化酶活性 (U/mg)显著升高 (82 4± 10 8,10 2 9± 76 ;P <0 0 5 )、胶原纤维相对含量 (% )显著降低 (2 2 30± 3 86vs.14 70± 2 0 7;P <0 0 5 ) ,肝硬化大鼠的门静脉压 (cmH2 O)亦显著降低 (14 4± 2 0vs.9 3± 1 5 ;P <0 0 5 )。结论 药理剂量的rhGH能够促进肝硬化大鼠的白蛋白合成、改善肝细胞功能 ,有助于抑制肝组织纤维化、缓解门静脉压力。
Objective To explore the mechanism by which recombinant human growth hormone (rhGH) protects liver function and alleviates portal hypertension in rats with liver cirrhosis. Methods Male S.D. rats with thioacetamide-induced liver cirrhosis were randomly assigned to receive separately normal saline (NS, 0.5 ml) or rhGH(333 ng/kg body weight) daily by subcutaneous injection for up to 7 days. After the respective treatments, changes of GH-binding capacity (R T), GHRmRNA, relative content of collagen (RCC), malon-dialdehyde (MDA), superoxide dismutase (SOD) in liver tissue, serum albumin and ALT and portal vein pressure (PVP) were examined. Results R T (fmol/mg protein) of GHR was respectively 31±4, 40±7(P<0.05), GHRmRNA (iOD,pixel) was 23±3, 42±8(P<0.05), MDA (nmol/mg protein) was 18.7±3.2, 12.0±2.2(P<0.05), SOD(U/mg protein) was 824±108, 1 029±76( P<0.05),RCC (%) was 22.30±3.86, 14.70±2.07(P<0.05) and serum albumin (g/L) was 29±4, 37±7(P<0.05), ALT (U/L) was 89±15, 69±7(P<0.05), and PVP(cm H 2O) was 14.4±2.0, 9.3±1.5 (P<0.05). Conclusions The expression of GHR and its mRNA were up-regulated by pharmacological dose of rhGH. The promotion of albumin synthesis, amelioration of liver functions, repression of fibrosis and remission of portal vein pressure were induced by administration of rhGH as a hepatotropic factor.
出处
《中华普通外科杂志》
CSCD
北大核心
2003年第8期474-476,共3页
Chinese Journal of General Surgery
基金
广东省自然科学基金资助项目 ( 9842 13 )
中山医科大学 2 11工程重点学科建设基金资助项目 (F0 0 0 0 990 75 )
关键词
生长激素
抗肝纤维化作用
门静脉高血压
肝硬化
Cirrhosis
Fibrosis, hepatic
Portal hypertension
Human growth hormone, recombinant
