摘要
为研究调心方 (主药 :党参、桂枝、茯苓、菖蒲、远志 )治疗阿尔茨海默病 (AD)的机理 ,将SD雄性大鼠脑室内注射DHF FeCl3 ADP ,造成氧化损伤型类AD模型 (造模前 1周起以调心方灌胃 ,每日 1次 ,连续 4周 ) ;采用Clark氧电极法观察鼠脑线粒体呼吸链氧化酶活性 ;HPLC 荧光法观察鼠脑氨基酸递质含量 ;RT PCR法观察大脑皮质NMDAR(NR1a和NR2 a)mRNA表达 ,以及调心方对AD大鼠上述指标变化的影响 ,并与Donepezil治疗组比较。结果 :模型大鼠脑组织氨基酸递质含量和NMDAR(NR1a和NR2 a)mRNA表达高于正常对照组 ,而线粒体呼吸链细胞色素氧化酶活性则显著下降 ;调心方对上述各项指标有不同程度的纠正作用。提示调心方可以通过纠正AD模型鼠脑组织呼吸链氧化酶活性和氨基酸递质的异常变化 ,这可能是该方防治AD的机理之一。
To study the mechanism of 'Heart-Regulating Decoction' in the treatment of Alzheimer's disease (AD), SD male rats were modeled to be of AD by intracerebral injection of DHF-FeCl 3-ADP. Before modeled, 'Heart-Regulating Decoction' was given by gastric perfusion, once a day and for 4 weeks. The respiration-chain oxidase activity, amino acid transmitter and the mRNA expression of NMDAR were measured; moreover, Donepezil was used as control. Results: In model group, the content of amino acid transmitter and the mRNA expression of NMDAR increased, higher than those in normal group, while the mitochondrion respiration-chain cytochrome oxidase reduced obviously; and the Decoction could correct them to different extents. It is indicated that 'Heart-Regulating Decoction' can correct the abnormal changes in the respiration-chain oxidase activity and amino acid transmitter, this may be one of its mechanisms in the prevention and treatment of AD.
出处
《上海中医药大学学报》
CAS
2003年第3期39-41,共3页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金重点项目 (39830 4 5 0 )
关键词
调心方
AD模型鼠
脑组织
呼吸链氧化酶活性
氨基酸递质
阿尔茨海默病
Alzheimer's disease (AD)
amino acid transmitter
glutamic acid receptor
cytochrome oxidase
'Heart-Regulating Decoction
