摘要
采用胶原酶、250μm、40μm筛和亲和层析技术,自人子宫内膜组织提纯子宫内膜抗原。SDS-PAGE分析见分子量为46、36和25KD的三条区带;过碘酸-Schiff试剂染色证实为糖蛋白。高压液相色谱层析示子宫内膜抗原富含16种氨基酸。各类元素和果糖测定结果表明,子宫内膜抗原含5种元素,不含果糖。
Using collagenase (Type Ⅰ) , 250μm, 40μm sieves and affinity chroma-tography, endometrial antigens were purified from human endometrial tissues. There were three bands with molecular weights (MW) 46KD, 36KD and 25KD on SDS-PAGE. Glycoprotein bands were confirmed by periodic acid-Schiffs staining. Results indicated that endometrial antigens were rich in 16 amino acids with HP 1090 Liquid-Chro-omatograph. Analysing of elements and fructose showed that there were 5 elements in endomatrial antigens, but no fructose.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第3期182-184,共3页
Immunological Journal
基金
国家自然科学基金资助项目
关键词
子宫内膜
子宫内膜抗原
Endometrium, Endometrial antigen (EMAg)
