摘要
目的观察诱导型一氧化氮合酶抑制剂-氨基胍(AG)对大鼠脊髓损伤后运动功能的影响。方法大鼠脊髓压迫伤后给予AG进行治疗,24 h后用分光光度法测定脊髓组织中一氧化氮(NO)含量和NOS活性;72 h后用流式细胞仪检测神经细胞凋亡情况;4周后用电生理和动物行为学等指标评价运动功能的恢复情况。以正常大鼠和损伤未治大鼠为对照。结果AG可以抑制组织中的NO含量和NOS活性,同时降低神经细胞的凋亡比率,提高动物后肢运动功能评分,恢复运动诱发电位的振幅和潜伏期,与对照及损伤未治大鼠相比均有显著差异(P<0.05)。结论脊髓损伤后应用NOS抑制剂可以使伤后运动功能得到改善,提示iNOS活性变化可能对脊髓损伤的恢复更具决定作用,其作用机制与抑制伤后细胞凋亡有关。
Objective To study the effects of aminoguanidine (AG), the inhibitor of inducible nitric oxide synthase (NOS), on the recovery of rat hindlimb motor function after spinal cord injury and explore the possible mechanism. Methods Thirty rat models of spinal cord compression injury were established according to Nystrom's method. AG therapy was administered for 4 times at 1 h before and 8, 24, 36 h after the injury, and 24 h after the completion of the therapy, spectrophotography was per-formed to measure the content of NO and activity of NOS in the injured spinal cord, followed by flow cytomertry for deter-mining the apoptotic rate 48 h later. The evaluation of the hindlimb motor function recovery was conducted by electrophysio-logical method and by measuring the behavior scores. Results AG significantly decreased the NO content (from 2.2714±0.4239 μmol/g·pro to 0.8466±0.0477 μmol/g×pro, P<0.05) and NOS activity (from 0.3408±0.0228 U/mg·pro to 0.2702±0.0148 U/mg·pro, P<0.05) in the injured spinal cord. The apoptotic rats were also reduced (from 7.88%±0.79% to 3.10%±0.66%, P<0.05). Four weeks after the therapy, the behavior score of the rats improved from 7.1±4.5 to 17.3±4.7 (P<0.01), and the la-tency and amplitude of the motor evoked potentials improved from 0 ms to 8.89±0.91 ms and from 0 mv to 1.99±0.48 mv re-spectively, showing significant therapeutic effect of AG (P<0.05). Conclusion AG can improve motor functions of injured spinal cord in rats, possibly resulting from decreased apoptotic cells of the neurons in the spinal cord in the early stages of the injury.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第7期687-689,共3页
Journal of First Military Medical University
基金
国家自然科学基金(39800166)~~
关键词
脊髓压迫伤
氨基胍
后肢运动功能
诱导型一氧化氮合酶抑制剂
病理生理学
spinal cord injuries/physiopathology
nitric oxide synthase/ antagonists and inhibitors
evoked potentials, motor/ drug effect
cell apoptosis
aminoguanidine/therapeutic use
