摘要
目的 探讨实用有效的普通狨猴血清酶学检验方法。方法 常规法分离血清,采用2,4-二硝基苯腙比色法,24 h内测定狨猴血清中异柠檬酸脱氢酶(ICD)活力,IFCC法测定丙氨酸转氨酶(ALT)、草氨酸转氨酶(AST)及γ-谷氨酰转肽酶(γ-GT)的活力,平板琼脂糖凝胶电泳测定乳酸脱氢酶(LDH)同工酶的活力水平,并与国内外相关实验结果进行比较。结果 75只普通狨猴的ALT、AST、γ-CT和ICD活力水平与国内外相关实验结果基本相符,不同试剂等实验条件会造成实验结果出现较大差异。结论 ALT、AST和ICD是甲型肝炎研究中狨猴肝脏损伤的有效指标。采用动物实验前自身对照值是可靠和科学的血清酶学分析方法。
Objective To develop a practical and effective method for enzymological analysis of marmoset serum.Methods Separate marmoset sera by routine method and detect the activities of ICD by 2,4-di-nitrophenylhydrazone colorimetric method, ALT, AST and 7-GT by IFCC method,and the isoenzyme of LDH by agarose gel electrophoresis on a plate.The results were compared with those reported abroad arid in China.Results The activities of ALT, AST,r-GT and ICD of 75 marmosets were basically consistent with those reported.The results obtained under different conditions were significantly different. Conclusion ALT, AST and ICD were valuable to the evaludon of liver lesion of marmoset. Using the activities of the enzymes before teat as control was reliable and scientific.
出处
《中国生物制品学杂志》
CAS
CSCD
2003年第5期279-281,共3页
Chinese Journal of Biologicals
基金
美国中华医学基金会(CMB)资助(编号93-582)
