摘要
研究探讨了不同取材时间、不同抗褐化剂及低温处理对紫枝玫槐外植体褐变的影响,结果表明：①取材时间尽量避免高温季节,以3-5月为宜;②在培养基中分别添加PVP、Vc、AC三种抗褐化剂,其中AC可有效抑制紫枝玫瑰茎段的褐化,同时促进了外植体的生长;③对外植体进行适当时间的低温处理可减轻外植体的褐变。
Experiment was made on effect of different stem collected seasons, treatment by different inhibitors and low temperature on browning of Rosa rugosa ＇Purple Branch＇ explant. The result showed that the suitable stem collect season was from March to May. Activated carbon supplemented in culture medium could effectively inhibit browning of the stem, and promote the growth of explant. Treatment of low temperature of explant could reduce browning.
出处
《浙江林业科技》
北大核心
2007年第5期41-43,共3页
Journal of Zhejiang Forestry Science and Technology
关键词
紫枝玫瑰
组织培养
褐化
Rosa rugosa ＇Purple Branch＇
tissue culture
browning
