摘要
目的 探讨大鼠烫伤后早期应用重组人生长激素 (rhGH) ,机体T细胞免疫和炎症反应的变化。 方法 将SD大鼠制成 30 %TBSA的Ⅲ度烫伤模型 ,随机分为用药组 :从伤后第 1天开始 ,腹部皮下注射rhGH 6U/kg ,连续 10d ;未用药组 :烫伤后不予用药 ;对照组 :不予烫伤。各组大鼠于伤后 3、6、10d留取血液及脾脏标本 ,检测肿瘤坏死因子α(TNFα)、白细胞介素 (IL) 2、IL 6浓度和CD4 + 、CD8+ 细胞数。 结果 未用药组大鼠伤后 3d ,TNFα、IL 2、IL 6浓度和CD4 + 、CD8+ 细胞数均显著增高 ,伤后 6d均明显下降。用药组伤后 3dCD4 + 、CD8+ 细胞显著增加 ,IL 2、IL 6和TNFα水平显著下降 ;伤后 6d ,用药组IL 2、IL 6水平上升 ,与对照组比较差异无显著性意义 ,TNFα水平明显高于对照组和未用药组。伤后 10d ,用药组IL 2、IL 6和TNFα水平仍呈逐渐上升趋势 ,未用药组IL 2、IL 6水平显著下降 ,TNFα水平有所上升。 结论 在烫伤后不同应激状态下应用rhGH ,对T细胞免疫和炎症反应可能产生不同影响。
Objective To investigate the changes in the systemic inflammatory response and T cell induced immunity after systemic administration of recombinant human growth hormone (rhGH) during early postburn stage. Methods Forty Spregue-Dawley (SD) rats were randomly divided into three groups for the study. The rats in control group (C, n=6) were only used for the determination of plasma levels of the cytokines, such as TNFα, IL 2, IL 6 and CD4 + and CD8 + cells. The SD rats in burn with rhGH treatment group (BT, n=18) and burn without treament group (B, n=18) were inflicted with Ⅲ degree scalding injury on the back. rhGH was injected subcutaneously on the abdomen of the rats in a dose of 6 IU/kg for 10 days in BT group. The blood samples were harvested from the rats in the two groups for the evaluation of the above indices. Results The plasma levels of TNFα, IL 2, IL 6 and CD4 + and CD8 + cells were increased on the 3 rd postburn day (PBD) and decreased on the 6 th PBD in B group,while the CD4 + and CD8 + cells were increased significantly and the plasma levels of TNFα, IL 2, IL 6 decreased obviously on the 3 rd PBD in BT group. And the plasma levels of IL 2 and IL 6 in BT group on the 6 th PBD showed no difference from those in C group. But the plasma TNFα level in BT group was evidently higher than that in B and C group on the 6 th PBD. Furthermore, the plasma levels of TNFα, IL 2 and IL 6 in BT group were still increased gradually on the 10 th PBD, while the IL 2 and IL 6 levels were decreased obviously in B group,but the TNFα level was increased. Conclusion Systemic administration of rhGH during different states of stress exerted different effects on T cell induced immunity and systemic inflammatory response. [
出处
《中华烧伤杂志》
CAS
CSCD
2003年第4期216-218,共3页
Chinese Journal of Burns
关键词
大鼠
烫伤
临床应用
重组人生长激素
炎症反应
动物模型
Recombinant human growth hormones
Systemic inflammatory response syndrome
T cell Immunity
Immune,cell
