摘要
对照尼氏图分析比较各品系小鼠G带核型400多个。胰酶处理的同一个细胞中期染色体的带没有尼氏图的那么多。不同细胞中带最多的染色体,其带的数目和位置与尼氏图基本一致。介绍了尼氏图小鼠区、带模式图及其命名系统;描述了各号染色体区、带特点及鉴别要点,并提供大带细胞区模式图和6种变异图型。讨论了正确分析核型的要点。
It is necessary to determine the position of break points in order to study the structure rearrangement of chromosomes and gene mapping. Therefore it is indispensable to set up region, banded idiogram and nomenclature system. Nesbitt and Franke reported an idiogram and a nomenclature system for band patterns of mouse chromosomes which thereafter was accepted by the Committee on Standardized Genetic Nomenclture. Nevertheless, there are few data of systematical contrast studies in this domain. There are still difficulties for distinguishing between some chromosomes because they are similar and variability. In this paper, we compared our G-banding karyotypes with the banding patterns of Nesbitt's idiogram. Our 400 karyotypes come from bone marrow cells and fibroblasts of several normal inbred and outbred mice. Each banding obtained from each well banded chromosomes of different cells were contrasted to that of Nesbitt's idiogram. The results are as follows: 1. we have not found so many bands in one metaphase chromosome banded with trypsin-Giemsa technique as reported by Nesbitt. 2. The banding numbers and positions on each well banding chromosome picked out from different metaphase plates were consistent with those of Nesbitt's idiogrom. But some bands such as 1E1 and 1E2; 2E5-2F5; 4A3-4A5; 5C1-5C3 can't usually be distinguished in our data. 3. We observed that the major banding structure of each karyotype remains stability, but the minor banding structure appears to be variablitity in inbred mice. In addition, 6 variant pictures and region idiograms in the G-banding structure of each chromosome were showed and some notices for correct analysis of mouse karyotype were discussed.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第3期309-315,共7页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
小鼠核型
中期染色体
G带模式图
Mouse karyotype
Metaphase chromosome
G-banding idiogram
