摘要
向猫盆神经注入10％蓖麻毒素2μl,存活3～5天后,再注射20％HRP溶液于同侧臂旁外侧核,2～3天后多聚甲醛和戊二醛灌注动物及取材。电镜下发现,盆神经初级传入纤维的终末(溃变)与向臂旁外侧核投射的位于骶髓后连合核、中间带外侧核、后角Ⅰ层内的HRP逆行标记神经元,形成轴-树和轴-体突触,从而在超微水平确证了盆内脏感觉传入的二级传导通路起源于骶髓后连合核、中间带外侧核、后角Ⅰ层,投射到臂旁外侧核。
2μl 10% ricin were injected into the pelvic nerve of cats. After 3～5 survival days 20% HRP solution was injected into the ipsilateral lateral parabrachial nucleus, 2～3 days later the animals were perfused with paraformaldehyde and glutaraldehyde, the brain and spinal cord were removed and processed for light and electron microscopy. It was confirmed that the primary afferent terminals (degenerated) of pelvic nerve formed axo-dendritic and axo-somatic synapses with the retrograde labelled neurons projected to the lateral parabrachial nucleus which were located in the dorsal commissure nucleus (DCN), intermediolateral nucleus (IML) and lamina Ⅰ of sacral spinal cord. Hence, the secondary afferent pathway of pelvic visceral sensation, as. indicated at the ultrastructural level, might originate from DCN, IML and lamina Ⅰ of sacral spinal cord and project to the lateral parabrachial nucleus.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第2期149-153,共5页
Acta Anatomica Sinica
关键词
超微结构
猫
盆内脏神经
二级传入
Secondary visceral afferent
Degeneration
HRP method
Ultrastructure
Sacral spinal cord
Cat
