摘要
本文应用免疫组织化学(PAP)方法,对猫骶髓“内脏面”(后连合核及其两侧的中间带)和Onuf核的L—ENK佯阳性成分的分布进行了观察。发现L—ENK样阳性纤维和终末布满于“内脏面”,特别是密布于后连合核、中间带内侧核、中间带外侧核及与其相邻的侧索。此外Onuf核也可见密布的纤维和终末。在经秋水仙素予处理的动物,L—ENK样细胞也出现于“内脏面”,但以中间带外侧核和后连合核两处较多。Onuf核及后根节均未见L—ENK样细胞。分别横断预、胸、腰髓后,后连合核、中间带外侧核及与其相邻的侧索等处L-ENK样阳性纤维和终末略有减少。切断一侧腰骶神经后根,L—ENK样阳性纤维和终末的分布未见变化。综合本文结果和文献资料,提示骶髓“内脏面”和Onuf核的L—ENK纤维和终末主要来源于脊髓内部固有的L—ENK样神经元;小部分来源于上位脑结构的下行投射。本文不支持来自后根节的周围性来源。
By using the PAP immunohistochemical method the distribution of leucine-enke phalin-immunoreactive (L-ENK-IR) elements in the sacral "visceral field" (dorsalcommissural nucleus and bilateral intermediate zone) and Onuf's nucleus of the cat have been examined. It has been found that the accumulation of L-ENK-IR fibers and terminals was densely concentrated in the DCN, intermediomedial nucleus (IMM), intermidiolateral nucleus (IML) and its adjacent lateral funiculus (LF). L-ENK-IR fibers and terminals appeared in low to middle density in the nucleus intercalatus(IC). In addition, high density of them was present in Onuf's nucleus, L-ENK-IR neurons were found in the "visceral field" , particularly in the DCN and IML of the colchicine-treated cats, But it was absent in the Onuf's nucleus and the sacral dorsal root ganglia.After transection at the cervical, thoracic and lunibar cord levels in different individuals of the cat, L-ENK-IR fibers and terminals in the IML and the part of LF near it and in the DCN were slightly dicreased. In the cats whose posterior roots (L7Co_1) has been cut, however, no change of the distribution of L-ENK-IR fibers and terminals was detected.Based on the present results and previous studies, it is concluded that the mojority of L-ENK-IR fibers and terminals in these areas originated from "intrinsic" origins in the sacral cord, and a part of them was supraspinal in origin. The view that they are from the dorsal root ganglion was not supported in the present study.
出处
《神经解剖学杂志》
CSCD
北大核心
1990年第2期171-178,共8页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金
关键词
骶髓
L-ENK
免疫组织化学
leu-enkephalia
sacral cord
immunohistochemical method
cat
