摘要
目的 :制订青藤碱凝胶剂的质量控制标准。方法 :采用薄层色谱法及紫外分光光度法鉴别青藤碱 ;用反相高效液相色谱法测定凝胶中青藤碱的含量 ;固定相为十八烷基硅烷键合硅胶 ,流动相为甲醇 0 .0 1mol·L 1 NaH2 PO4 (体积比 5 0∶5 0 ) ,氯霉素作内标。结果 :薄层色谱斑点清晰 ,对照品与供试品斑点在同一位置 ;紫外最大吸收波长 2 63nm ,平均回收率在 ( 99.6± 1.3 ) %。结论 :该方法简便、快速、准确 。
Objective:To formulate a quality control standard for sinomenine gel. Methods:TLC and UV spectrophotometry were employed for the identification of sinomenine. The content of sinomenine in the gel was determined with RP HPLC(Reversed phase high performance liquid chromatography). The column was composed of packed ODS C 18 and a methanol pH 6.8, 0.01 mol·L 1 NaH 2PO 4(50∶50 by volume) mixture served as the mobile phase. Chloramphenicol was used as the internal standard. Results:The TLC identification was highly specific and the spots were clear and concentrated. The spots of the control and test samples appeared on the same positions. The maximum UV absorption wavelength was 263nm, and the average rate of recovery was 99.6%±1.3%. Conclusion:The method was shown to be simple and convenient, rapid and accurate and suitable for the quality control of sinomenine gel.
出处
《医药导报》
CAS
2003年第8期571-572,共2页
Herald of Medicine
