摘要
目的:研究青藤碱贴剂在病理状态下的药动学规律。方法:以佐剂性关节炎大鼠为研究对象,以HPLC-UV为检测手段,测定给药以后不同时间点的血药浓度,计算药动学参数。结果:建立了专属性的血药浓度检测方法,青藤碱贴剂达峰时间约10h,MRT约24h,达峰浓度约455ng/mL,其体内行为属于一级速率、二室开放模型。结论:该方法灵敏、快速、正确、选择性强,较好的满足青藤碱的药动学研究,为合理用药提供参考。
Objective： To explore the pharmacokinetic of Sinomenine transdermal patch. Method： The plasma drug concentration of rats arthritis model induced by Freund＇s adjuvant was determined after administration with HPLC-UVD as analysis tools. The pharmacokinetics parameters was fitted with kinetica software package. Result： The exclusive analysis method was established with the following pharmacokinetics parameters： T（peak） = 10 h,Cmax = 455 ng /mL,MRT = 24 h. The pharmacokinetic characteristics was accordance with one-order rate and two – compartment model. Conclusion：The method is suitable to be applied to the pharmacokinetics research and further applied pharmacological study which will play a reference role in clinical application.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第5期123-126,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金资助项目(30672669)
中国博士后基金资助项目(20070420509)
关键词
青藤碱贴剂
佐剂性关节炎大鼠
药动学
Sinomenine patch
arthritis rats model induced by Freund＇s adjuvant
pharmacokinetics
