摘要
应用低温辐射技术诱导不同亲疏水性高分子玻璃态单体聚合固定葡萄糖氧化酶,研究不同实验条件对辐射聚合固定化葡萄糖氧化酶相对活性的影响。结果表明:在四种不同亲疏水程度的玻璃态高分子单体(2-hydroxyethyl methylacylate,polyethylene glycol dimethacrylate 9G,4G,2G)中,亲水性的HEMA、9G的固定化效果较好;在高分子单体浓度为50％,相应的聚合物载体空孔率为30％时,固定化葡萄糖氧化酶与底物10次反应的平均活性产率最高,达62％左右;当聚合物单体的冷却速率为70℃/min左右,辐照温度为—78℃,辐照剂量为1×10~4Gy,pH为5.4时,可以得到活性较高的固定化葡萄糖氧化酶。同时还对上述影响固定化葡萄糖氧化酶相对活性的原因进行了探讨。
This study was carried out to reveal the effects of various experimental conditions on the relative activity of glucose oxidase immobilized by 6(Co-Y radiation-induced polymerization at low temperatures by means of hydrophilic and hydrophobic glass-forming monomers ,i. e. , 2-hydroxyethyl methylacrylate (HEMA) and a series of polyethlene glycol dimethacrylate(9G, 4G,2G). The results showed that the relative activity of immobilized glucose oxidase varied with irradiation dose,monomers,monomer concentration,pH,irradiation temperature,cooling rate of the monomeric system and porosity of the polymer matrix. The optimum conditions for the immobilized glucose oxidase were irradiation dose 104 Gy, monomer concentration 50% (polymer porosity 30%), pH 5. 4 and polymerization temperature - 78C. One of the important factors affecting the relative activity of immobilized glucose oxidase in this study was the type of the monomers. It was found that the relative activity of glucose oxidase immobilized by means of hydrophilic glass-forming monomers was higher than that by means of hydrophobic glass-forming monomers and the former could be used more than 10 times. Another key factor in determining the average activity yield of immobilized glucose oxidase was the monomer concentration in monomer-buffer solution, which also showed a direct effect on the structure of the polymer. Reduction in the cooling rate of a monomeric system and in the monomer concentration had the same effect on porosity of the polymer and both lowered the relative activity of immobilized glucose oxidase.
出处
《江苏农业学报》
CSCD
1992年第4期35-39,共5页
Jiangsu Journal of Agricultural Sciences
关键词
葡萄糖
氧化酶
低温辐射
诱导
聚合
Immobilized enzyme
Radiation-induced polymerization
Glucose oxidase
