摘要
在低温（－７８℃）条件下，用６０Ｃｏγ射线对单体丙烯酸羟乙酯（ＨＥＡ）与聚乙二醇二甲基丙烯酸酯系列单体１４Ｇ、９Ｇ、４Ｇ、２Ｇ等进行辐射引发共聚，获得了亲水性共聚合凝胶载体，采用这些载体吸附固定化酒精酵母细胞的乙醇产率均高于游离细胞，其中Ｐｏｌｙ（ＨＥＡ—１４Ｇ）共聚物载体乙醇产率是游离细胞的２．４５倍，１２次分批反应均高于游离细胞。实验结果表明：固定化酵母细胞的乙醇生产率与共聚体系内亲水性单体及其它单体的浓度配比、双官能团单体的链长、共聚物载体的水合度以及载体结构、多孔性因素等有关，乙醇生产率的高低取决于载体的膨胀能力和多孔性．
Hydrophilic monomer 2-hydroxyethyl acrylate(HEA) and a series of polyethylene glycol dimethacrylate monomers were copolymerised by radiation technique at low temperature(-78℃) and hydrophilic were obtained.The immobilization of yeast cells with these copolymeried carriers led to a higher ethanol productivity than free cells.Of all copolymer carriers,the ethanol yield with poly(HEA-14G) was the highest,about 2.45 times as high as that of free yeast cells.In addition, the ethanol productivity of 12 batch repeated reactions with poly(HEA-14G) carrier was all higher than that of free yeast cells.The ethanol productivity of immobilized yeast cells was dependent on the proportion of hydrophilic monomer to other monomers in copolymer systems,the chain length of the bifunctional monomer,the degree of hydration of copolymer carriers,the structure of copolymer carriers and porosity in the internal structure of carriers.The ethanol yield of immobilized cells depended on swelling ability and porosity of copolymer carriers.
出处
《核技术》
CAS
CSCD
北大核心
1994年第2期119-124,共6页
Nuclear Techniques
关键词
固定化
酵母细胞
辐射聚合
Radiation copolymerization
Immobilized yeast cells
Hydrophilic hydrogel carriers
