摘要
目的 :探讨p38MAPK在介导TNF α所致大鼠胶质瘤细胞C6凋亡中的作用。方法 :应用MTT法检测TNF α处理的C6细胞的增殖活性 ,采用透射电镜和流式细胞仪观察凋亡的发生 ,应用SABC法和Westernblot检测p38MAPK的表达 ,应用流式细胞仪及SABC法观察 p38MAPK特异性抑制剂SB2 0 2 1 90对TNF α诱导C6细胞凋亡的影响。结果 :TNF α(2× 1 0 5U/L)对C6细胞增殖的抑制率为 43 .75 % ,透射电镜下可见典型的凋亡细胞 ,流式细胞仪检测凋亡率为 37.5 % ,SABC法和Westernblot显示P38MAPK表达阳性 ;加入SB2 0 2 1 90后 ,其凋亡率为 7.0 % ,未见P38MAPK表达。结论 :TNF α能诱导C6细胞凋亡和 p38MAPK表达 。
Purpose:To study the role of p38MAPK in mediating TNF α induced apoptosis in rat glioma cells C6.Methods:The proliferation activity of C6 cells after the treatment by TNF α was observed by MTT assay. The TNF α induced apoptosis was detected by transmission electron microscopy and flow cytometry. The expression of p38MAPK was detected by SABC method and Westernblot. The effect of SB202190, a specific inhibitor of p38MAPK on TNF α induced apoptosis was observed by flow cytometry and SABC method. Results:The inhibitory rate of TNF α (2×10 5 U/L) on C6 cells was 43.75%. In the TNF α treated group, apoptotic cells were observed by transmission electron microscopy and the apoptotic rate was 37.5% by flow cytometry, p38MAPK positive signals were found by SABC method and Westernblot. In the SB202190 treated group, the apoptotic rate was 7.0% and no p38MAPK signals were found.Conclusions:The apoptosis of C6 cells and expression of p38MAPK could be induced by TNF α. The activation of p38MAPK promoted the apoptosis of C6 cells. [
出处
《中国癌症杂志》
CAS
CSCD
2003年第3期215-217,228,共4页
China Oncology
