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苯乙酸对胶质瘤细胞C6增殖的抑制及对P16基因的激活作用 被引量：1

Growth inhibitory and differentiation inducing activity of phenylacetate and increased effect of p16 gene on glioma cell C6
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摘要 目的 :观察诱导分化剂苯乙酸对胶质瘤细胞C6增殖的抑制作用 ,及对抑癌基因p16的蛋白水平表达变化的影响。方法 :[3H] TdR掺入法测细胞增殖率 ;免疫组化SP法检测p16基因蛋白水平的表达。结果 :应用苯乙酸后 ,细胞CPM值降低 ;p16基因胞浆阳性表达显著升高。结论 :苯乙酸对胶质瘤细胞存在剂量依赖性抑制 ,增强了抑癌基因p16表达活性 。 Purpose:To determine the growth inhibitory and differentiation induing activity of phenylacetate (PA) on glioma cell line C6, to find the change of P16 gene expression. Methods:The effect of PA on cell proliferation using TdR assay,P16 gene protein expression studied by immunohistochemistry technique.Results:At does ranges 0.625、2.5、5.0 mmol/L,PA caused a dose dependent proliferation inhibitory effect. The protein expression of p16 gene was notably increased by PA. Conclusions:PA caused a does dependent proliferation inhibitory effect, the expression of p16 gene activity increased by PA.
作者 田宇 陈宝琴 赵兴利 田洋
机构地区 白求恩医科大学三院神经外科 吉林省人民医院 吉林省中医中药研究院
出处 《中国癌症杂志》 CAS CSCD 2000年第3期205-206,共2页 China Oncology
基金 国家自然科学基金 !(编号 3990 0 15 2 ) 吉林省科委项目 !(编号 970 5 6 7 4)
关键词 苯乙酸 胶质瘤 P16基因 免疫组化 细胞增殖 phenylacetate glioma p16 gene immunohistochemial
分类号 R730.264 [医药卫生—肿瘤]
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参考文献4

  • 1AlainThibault,MichealCooper,WilliamFigg,etal.APhaseⅠandpharmacokineticstudyofintravenousphenylacetateinpatientswithCancer[J].CancerResearch.1994,54(18):1690-1694.
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  • 3田宇,陈宝琴,王校复,李凡,周建.苯乙酸对胶质瘤细胞C_6DNA合成的抑制作用[J].白求恩医科大学学报,1999,25(1):32-34. 被引量：4
  • 4HamaS,HeikeY,TakahashiM,etal.Adenovirus-mediatedp16genetransferdrug-inducedcelldeaththroughG1arrestinhumangliomacell[J].IntJCancer,1998,77(1):47-54.

二级参考文献1

  • 1张友会，现代肿瘤学.基础部分，1993年，42页

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中国癌症杂志
2000年 第3期

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