摘要
目的 观察诱导分化剂苯乙酸对胶质瘤细胞Ｃ６的增殖分化过程中癌基因Ｃ－ｍｙｃ的蛋白水平变化。方法 〖＾３Ｈ〗ＴｄＲ掺入法测细胞增殖率，免疫组化ＳＰ法检测Ｃ－ｍｙｃ基因蛋白水平的表达。结果 应用苯乙酸后，细胞ＣＰＭ降低；Ｃ－ｍｙｃ基因胞浆阳性表达显著下降。结论 苯乙酸对胶质瘤细胞存在剂量依赖性抑制作用机制可能为直接抑制胞浆中ＲＮＡ合成。
Objective To determine the growth-inhibitory and differentiation-induing activity of phenylacetate (PA) on glioma cell C6. To find the mechanism of PA on molecule level.Methods The effect of PA on cell proliferation was deter-- mined by [~3H]-TdR assay; cell cycle distribution was analyzed with flow cytometry; and C-myc gene protein expression was studied by immunohistochemial technique.Results At doses of ranging 0.625,2.5,5.0mM, PA caused a dose-dependent prolif-- eration inhibitory effect. Cells arrested in Gall, Phases were observed in PA--treated (2.5.5.0mM) cells. The protein expres- sion of C--myc gene was notably decreased by PA (0. 625,2. 5mM). Conclusion PA caused a dose--dependent proliferation in - hibitory effect. One of the mechanisms of the effect should be PA decreased RNA amount directly in cells, although a lot of cells entered S phase, but most of them can not finish cell cycle. All of these effects connected with C-myc gene pro- tein expression were deceased by PA.
出处
《中国实验诊断学》
2000年第2期57-59,共3页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金!(编号39900152)
吉林省科委!(970567-4)
