摘要
以芸芥为材料 ,分别用CTAB法、SDS法、尿素法、NaOH法四种方法对芸芥基因组DNA进行了提取 ;并用紫外光分光光度计法、琼脂糖电泳法和RAPD分析法对所提取的DNA进行检测 ,将它们在DNA的产量、质量和耗时、耗费等方面的优缺点进行比较 ,以便在实际工作中根据不同的试验条件选取最合适的提取方法。通过四种方法的比较 ,研究认为尿素法是芸芥基因组DNA的最佳提取方法。
methods——CTAB method, SDS method, urea method, and alkali method were used to extract genomic DNA from the leaves of E.sativa Mill. The resulted DNA samples of the methods were tested using agarose gel electrophoresis and PCR-RAPD. The difference in yield, quality, time duration and expense of the obtained DNA were compared, and urea method was decided as the best method for the test.
出处
《生物技术通报》
CAS
CSCD
2003年第3期44-47,共4页
Biotechnology Bulletin
