摘要
通过比较文献中不同的色谱条件及样品处理过程,建立了测定鱼组织中喹乙醇残留量的高效液相色谱法。该法采用C_(18)色谱柱,选择甲醇—三蒸水(体积比15:85)为流动相,372 nm为检测波长,样品用150 g/L的三氯乙酸沉淀蛋白,经15 000 r/min离心取上清液进样。喹乙醇在0.2～12.8 μg/g范围内线性关系良好,检测限为0.04 μg/g,平均回收率为85.93％,不同浓度水平的日内和日间测定的相对标准差均小于10％。
Through the comparison of different chromatographic conditions and sample treatment processes, the residue of olaquindox in fish tissues was determined by high performance liquid chroma-tography(HPLC) . It used a C18 column,and the mobile phase consisted of methanol tridistilled-water (volume ratio 15 : 85). The UV detection wavelength was 372 nm. Samples were deproteined with 150 g/L trichloroacetic acid . The precipitated mixture was shaken and then centrifuged, the filtrate was evaporated to dryness. Within the range of 0. 2-12. 8 μg/g,olaquindox had a good linearity. The detection limit was 0. 04 μg/g and the mean recovery of olaquindox was 85. 93%. The intra-day and inter-day precision expressed by RSD was less than 10% at three drug levels.
出处
《湖北农学院学报》
2003年第4期266-270,共5页
Journal of Hubei Agricultural College
基金
农业部重点科研资助项目(渔95-B-00-01-03)
关键词
鱼组织
喹乙醇
残留量
高效液相色谱
检测方法
fish tissues
olaquindox residue
high performance liquid chromatography
