摘要
目的 :建立C型利钠肽 (CNP)放射免疫分析 ,并初步探讨其临床应用价值。方法 :将甲状腺球蛋白与CNP联接 ,免疫家兔制备抗体。建成CNP放射免疫分析法 ,研制成功放免试剂盒。应用本试剂盒测定83例健康人、5 4例冠心病、2 5例心力衰竭、73例高血压患者血浆CNP水平。结果 :分析灵敏度为 5pg/ml。所获CNP抗血清特异性强 ,与心钠素 (ANP)、神经降压素 (NT)、神经肽Y(NPY)、内皮素 (ET)、降钙素基因相关肽(CGRP)、降钙素 (CT)等多肽均无交叉反应。批内变异系数 <10 % ,批间变异系数 <15 %。心力衰竭、冠心病、高血压患者血浆CNP浓度分别为 6 2 5pg/ml、4 7 9pg/ml、5 6 4pg/ml,明显高于健康人 (2 7 5pg/ml)。结论 :CNP放射免疫分析操作简便、快速 ,为进一步研究CNP在人体生理、病理状态及基础医学研究工作提供了一种可靠的手段。
Objective To develop a RIA kit for measurement of C-type natriuretic peptide (CNP) and to investigate its in clinical use. Methods Conjugated CNP-TG was used to immunize the rabbit and the antibody against CNP was prepared. CNP RIA kits were made from it. Plasma CNP concentrations of 83 controls, 54 patients with coronary heart disease, 25 patients with heart failure and 73 patients with primary hypertension were determined with this RIA kit. Results The sensitivity of detection was 5pg/ml. The specificity of CNP anti-serum was high and did not react with ANP, NT, NPY, ET, CGRP and CT. The intra and interassy CVs were<10% and <15% respectively. The plasma levels of patients with coronary heart disease, heart failure and primary hypertension were 62.5pg/ml, 47.9pg/ml, 56 4pg/ml respectively. There were all significantly higher than the level in controls (27.5pg/ml). Conclusion RIA of CNP with this kit is a simple, rapid method. It is a reliable means for studying the role of CNP in various physiological and pathophysiological states.
出处
《放射免疫学杂志》
CAS
2003年第3期136-138,共3页
Journal of Radioimmanology
