摘要
目的 观察溴氰菊酯 (DM )对神经细胞游离钙浓度 ([Ca2 + ]i)及凋亡的影响。方法 Wistar大鼠随机分为对照组与 3个染毒组 ,染毒组腹腔注射 1 2 .5mg/kg的DM ;以Fura 2 /AM为荧光指示剂 ,RF 530 1PC荧光分光光度计测定染毒后 5、2 4、48h后大鼠神经细胞 [Ca2 + ]i浓度的变化 ;FACS42 0型流式细胞仪测定细胞凋亡率。结果 PM染毒 5、2 4、48h组大鼠海马及皮层细胞 [Ca2 + ]i分别为 :5h组海马 :(389.94± 43 .64)nmol/L、皮层 :(449.33± 2 3 .2 3)nmol/L ;2 4h组海马 :(340 .47±32 .36)nmol/L、皮层 :(31 1 .62± 2 5 .48)nmol/L ;48h组海马 (2 87.1 3± 2 4 .2 9)nmol/L、皮层 :(346 .55±36 .87)nmol/L ,均高于对照组 [海马 :(2 0 3 .2 4± 1 8.53)nmol/L、皮层 :(2 2 6 .85± 1 4 .81 )nmol/L] ,差异有显著性 (P <0 .0 1 )。染毒 48h组大鼠此二项指标值较 5h组明显下降 ,差异有显著性 (P <0 .0 5)。染毒 2 4、48h组大鼠神经细胞凋亡率 [海马 :(8.45± 1 .0 2 ) %、(9.44± 1 .1 4 ) % ,皮层 :(7.90± 0 .49) %、(8.0 1± 0 .87) % ]均明显高于对照组 [海马 :(2 .97± 0 .36) %、皮层 :(3 .50± 0 .48) % ] ,差异有显著性 (P<0 .0 1 ) ,且有时间 反应关系 (r=0 .940、0 .893)。结论 DM可影响大鼠神?
Objective To study the effects of deltamethrin on intracellular free Ca 2+ concentration and apoptosis in rat neural cells. Methods Wister rats were randomly divided into 4 groups(3 treated groups and 1 control) .Intracellular free Ca 2+ concentration in rat neural cells was measured by using the fluorescent Ca 2+ indicator Fura 2/AM.Apoptotic rate of neural cells was measured by using FACS420 Flow Cytometer. Results Intracellular free Ca 2+ concentration at 5 h after deltamethrin exposure[hippocampus:(389.94±43.64) nmol/L,cerebral cortex:(449.33±23.23) nmol/L],at 24 h[hippocampus:(340.47±32.36) nmol/L,cerebral cortex: (311.62 ±25.48) nmol/L] and at 48 h[hippocampus:(287.13±24.29) nmol/L,cerebral cortex:(346.55±36.87) nmol/L] were all higher than those of the control group[hippocampus:(203.24±18.53) nmol/L,cerebral cortex:(226.85±14.81) nmol/L, P <0.01];Apoptotic rate in neural cells 24 h and 48 h later[hippocampus:(8.45±1.02)%, (9.44 ±1.14)%,cerebral cortex: (7.90 ±0.49)%,(8.01± 0.87)%] were also higher than those of the control group[hippocampus:(2.97±0.36)%,cerebral cortex:(3.50±0.48)%, P < 0.01)] and increased with time prolonged. Conclusion Exposure to high dose of deltamethrin would interfere with intracellular free Ca 2+ concentration and apoptotic rate in rat neural cells,suggesting that there may be certain relation between them.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2002年第6期427-429,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
