摘要
目的：探讨槲皮素（Ｑｕｅ）对血管平滑肌细胞胞内游离钙浓度（［Ｃａ^（２＋）］ｉ）的影响。方法：采用新一代钙荧光探剂 Ｆｌｕｏ－３／ＡＭ检测 Ｑｕｅ对培养的兔主动脉血管平滑肌细胞（ＡＳＭＣ）［Ｃａ^（２＋）］ｉ在高Ｋ~＋、去甲肾上腺素（ＮＥ）、血管紧张素Ⅱ（ＡｎｇⅡ）刺激作用下的改变，并与Ｖｅｒａｐａｍｉｌ进行对照研究。结果：Ｑｕｅ（１０^（－６）、１０^（－５）、１０^（－４）ｍｏｌ／１）呈剂量依赖性抑制高Ｋ~＋去极化引起的［Ｃａ^（２＋）］ｉ升高，与Ｖｅｒａｐａｍｉｌ作用相似，但弱于Ｖｅ－ｒａｐａｍｉｌ；Ｑｕｅ（１０^（－６）～１０^（－４）ｍｏｌ／Ｌ）对ＮＥ，ＡｎｇⅡ通过受体介导引起的［Ｃａ^（２＋）］ｉ增高也具有明显的抑制作用。但Ｑｕｅ对静息状态的ＡＳＭＣ［Ｃａ^（２＋）］ｉ无明显影响。结论：Ｑｕｅ通过对血管平滑肌细胞电压依赖性钙通道和受体操纵性钙通道双重抑制作用，降低细胞内游离钙水平，这可能是其舒血管降压机制之一。
Aim: To explore the effects of quercetin (Que) on intracellular free calcium ([Ca^(2+)] i) in vascular smooth muscle cells. Methods: The effects of Que on changes in [Ca^(2+)]i were determined in cultured rabbit aortic smooth muscle cells (ASMC) after exposure to high K^+,norepinephrine (NE) and angiotensin Ⅱ (Ang Ⅱ ). Fluorescent Ca^(2+) -indicater fluo-3/ AM was used. The effects of Que were compared with that of verapamil. Results: It was found that one (10^(-6)- 10^(-4)mol/L) inhibited the elevation of [Ca^(2+)]i induced by high potassium- depolarization in a concentration dependent manner. These effects were similar to but weaker than those of verapamil. In addi- tion,it was shown that one (10^(-6) -10^(-4)mol/L) inhibited the elevation of [Ca^(2+)]i induced by NE and Aug Ⅱ in the presence of extracellular Ca^(2+). In the absence of extracellular Ca^(2+),Que (10^(-6)- 10^(-4))mol/L also had some blocking effects on the NE-induced [Ca^(2+)]i increase. Conclusions: The results suggest that Que mighit decrease the [Ca^(2+)]i of ASMC by blocking both voltage-dependent calcium channels and receptor-operated calcium channels,which is one of hypotensive mechanisms.
出处
《高血压杂志》
CSCD
2000年第1期55-57,共3页
Chinese Journal of Hypertension
关键词
槲皮素
血管平滑肌细胞
药理
胞内游离钙浓度
quercetin
vascular smooth muscle cells
intracellular free calcium
rabbit
