摘要
目的 探讨超氧化物歧化酶 (SOD)基因转染大鼠肝细胞对胆汁毒性损害的保护作用。方法 SOD基因转染培养的大鼠肝细胞 ,克隆、筛选及鉴定阳性克隆 ,PCR法、Western -blot印迹分析检测SOD基因表达 ,甲基偶氮唑蓝 (MTT)比色法检测SOD基因转染肝细胞对胆汁毒性损害的保护。结果 SOD基因转染肝细胞对于2 %浓度胆汁抑制率由 12h的 ( 78 80± 12 3 5 ) %下降到 ( 4 3 3 5± 9 69) % ,2 4h由 ( 82 5 5± 11 2 7) %下降到( -3 9 5 4± 15 13 ) % ,48h由 ( 83 83± 18 69) %下降到 ( -4 8 3 2± 15 2 5 ) % ,P <0 0 1。
Objective\ To investigate the protective effect on toxicity of bile through superoxide dismute (SOD) gene transfection hepatocyte of rat.Methods\ The recombinant of retrovirus and SOD gene (pLNCX-SOD) packaged with lipofection AMINE was transfected into the hepatocyte in vitro,which stably expressed SOD gene.The protective effects on bile toxicity were detected with MTT chromometry.Results\ After transfection,hepatocyte increased the protective effect on injury of bile toxicity.Inhibitory to bile with concentration of 2% decreased obviously from (78 80±12 35)% to (43 35±9 69)% in 12 hours,from (82 55±11 27)% to (-39 54±15 13)% in 24 hours,from (83 83±18 69)% to (-48 32±15 25)% in 48 hours,compared with that of the untransfected cell,P<0 01 Conclusion\ After transfection of pLNCX-SOD gene,transfected hepatocyte could obviously resist to the toxicity of the bile.
出处
《中国实用外科杂志》
CSCD
北大核心
2003年第6期346-347,共2页
Chinese Journal of Practical Surgery
