摘要
目的 探讨梗阻性黄疸 (简称梗黄 )大鼠心肌组织中TNF α及超氧化物歧化酶 (SOD)基因mRNA的表达。方法 RT PCR法扩增梗黄大鼠心肌组织中cDNA ,电泳扫描后半定量分析TNF α和SOD基因mRNA表达。结果 梗黄大鼠心肌组织中TNF α基因mRNA表达增强 ,SOD基因mRNA表达下降 ;血清中TNF α水平升高 ,心肌组织中SOD合成减少。结论 梗黄心肌的损害与TNF α基因mRNA表达增强。
Objective To investigate the expressions of TNF-α and superoxide dismutase (SOD) mRNA in myocardium of rats with obstructive jaundice (OJ). Methods The expressions of TNF-α and SOD mRNA were semi-quantitatively analyzed after amplification of cDNA in myocardium of the rats with OJ by RT-PCR. Results The expression of TNF-α mRNA increased and that of SOD mRNA decreased in the myocardium of rats with OJ. The level of plasm TNF-α increased and SOD synthesis in myocardium decreased. Conclusion The injury of myocardium in OJ is correlated with increasing expression of TNF-α mRNA and decreasing expression of SOD mRNA.
出处
《中国普外基础与临床杂志》
CAS
2004年第6期502-504,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
辽宁省教委科研基金项目 (项目编号 :2 0 2 2 0 3 2 5 7)~~
