摘要
研究了微乳液介质中,在pH=10.0硼砂 氢氧化钠缓冲溶液时,钴与苯基荧光酮(PF)显色生成稳定的1∶2配合物,在637nm处摩尔吸光系数为1.21×105L/(mol·cm),钴含量在0～1.0μg/mL范围内符合比耳定律,用所拟方法以巯基葡聚糖凝胶分离富集,可测定维生素B12中的微量钴,结果令人满意.
The color reaction of cobalt with phenylfluorone (PF) in microemulsion has been studied. Cobalt (Ⅱ) reacts with PF to form a stable 1∶2 complex at pH=10.0. Its apparent molar absorptivity is 1.21×10\+5 L/(mol·cm) at 637 nm. Beer's law is obeyed in the range of 0～1.0 μg/mL for cobalt (Ⅱ). The coexistent metal ions can be separated and cobalt can be enriched by sulphydryl dextrane gel (SDG), greatly improving the selectivity and sensibility of system. The method is used to determine cobalt(Ⅱ) in vitamin B\-12 samples with satisfactory results.
出处
《分析测试技术与仪器》
CAS
2003年第2期70-73,共4页
Analysis and Testing Technology and Instruments
基金
山东省自然科学基金资助 (编号 :Y2 0 0 0B0 9).
