摘要
目的:了解白细胞介素1基因家族[白细胞介素(interleukin,IL)1 α、IL-1β、IL-1受体拮抗剂(inter-leukine-1 receptor antagonist,IL-1RA)及IL-1β前体(interleukine-1β precursor)在翼状胬肉上皮内的表达。方法:用免疫荧光组织化学方法对10例初发性翼状胬肉患者切除的翼状胬肉组织中IL-1α、IL-1β、IL-1β前体及IL-1RA蛋白进行检测,并与正常人结膜组织进行对照。结果:免疫荧光组织化学染色显示,在正常结膜上皮中,IL-1α及IL-1β全层有弱阳性表达,上皮细胞表达较基底细胞明显,IL-1β前体只在上皮细胞表层表达,IL-1RA则无明显的表达。10例翼状胬肉患者中7例上皮的IL-1α、IL-1β、IL-1β前体及IL-1RA表达与正常结膜明显不同,表现为IL-1α、IL-1β、IL-1β前体较正常明显增加,IL-1α及IL-1β在全层呈强阳性,且基底细胞与上皮细胞的表达强度基本一致,IL-1RA表层细胞呈阳性表达,3例IL-1α、IL-1β、IL-1β前体及IL-1RA表达较正常结膜稍强。结论:翼状胬肉组织上皮IL-1α、IL-1β、IL-1β前体及IL-1RA表达增加,提示翼状胬肉可能处于轻微的非感染炎症状态。眼科学报2003;19:133-136。
Purpose:To evaluate the expression of interleukine 1 family including interleukin-1, (IL-1α)、interleukin-1β (IL-1β),interleukine-1 receptor antagonist, (IL-IRA) and interleukine-1β precursor by the epithelium in pterygium so as to understand the patho-genesis of this disorder.
Methods:. The immunoflurescent staining was performed in 10 patients with primary pterygium, compared to 10 normal conjunctival epithelium,to determine the expression of IL-1α, IL-1β, IL-1β RA and IL-1βprecursor proteins
Results:The IL-1α and IL-1 βwere present at whole epithelium while the IL-1βprecursor was expressed by the superficial cells in normal conjunctival epithelium in im-munofluorescent staining. No IL-1RA positive staining was found in normal conjunctiva. Of 10 pterygia, 7 pterygias were stained by IL-1αand IL-1β in the full thickness of epithelium, IL-1β precursor and IL-1RA in the superior cell and with strong staining compared to control group, 3 of them showed the similar staining pattern with normal conjunctiva.
Conclusion:The increasing IL-1α, IL-1β, IL-1β RA and IL-1βprecursor proteins are presented in pterygium, which shows that the pterygium is in the state of no-infective inflammation. Eye Science 2003; 19:133-136.
出处
《眼科学报》
2003年第2期133-136,共4页
Eye Science
