摘要
目的 制定益津降糖颗粒质量标准。方法 采用薄层色谱法定性鉴别甘草、仙人掌 ;用高效液相色谱法测定制剂中人参皂苷Rg1及Re的含量。以C18化学键合硅胶为固定相 ,以乙腈 0 .0 5 %磷酸溶液 (99∶ 4 0 0 )为流动相 ,检测波长为2 0 3nm。结果 定性鉴别色谱特征明显 ,重现性好 ;含量测定Rg1及Re的平均回收率分别为 97.6 9% ,98.35 % ,线性范围分别为 0 .6 2 8～ 4 .396 μg、0 .92 0～ 6 .4 4 0 μg。结论 建立了该制剂的质量标准 ,其定性与定量方法可靠。
OBJECTIVE To establish Quality standards of Yijinjiangtang Keli. METHOD Radix Glicyrrhizae and Herba Opuntia dillenii were identified by TLC. The content of Ginsenoside Rg1 and Re in Yijinjiangtang Keli were determined by HPLC, with C18 chemical bonded silica gel as solid phase and acetonitrile 0.05%phosphoric acid solution(99∶400)as mobile phase, UV detection wavelength at 203nm. RESULTS The characteristics for identification by TLC were distinct. The average recoverys of Rg1 and Re were 97.69% and 98.35%. Their linear range were 0.628～4.396μg and 0.920～6.440 μg respectively. CONCLUSION The quality standard of the preparation was provided. The method for identification and quantification was reproducible and realizable.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2003年第3期215-217,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
益津降糖颗粒
甘草
仙人掌
人参皂苷RG1
RE
Yijinjiangtang keli
Radix Glycyrrhizae
Herba Opuntia dillenii
GinsenosideRg1 and Re
