摘要
应用反相高效液相色谱法检测了雷公藤提取物中雷公藤甲素的含量。样品经洗脱提取后，以Ｃ１８化学键合硅胶为固定相，乙腈－水（４０∶６０，Ｖ／Ｖ）为流动相，用２１０ｎｍ波长定量检测。测定结果表明，雷公藤甲素浓度在０．２５～４．００ｍｇ／Ｌ范围内线性良好，最低进样检测浓度为０．１ｍｇ／Ｌ。批内（ｎ＝５）及批间（ｎ＝５）测定相对标准偏差分别为１．９％～６．５％和２．７％～７．８％，回收率为８９．８％～９２．０％。
A reversed phase liquid chromatographic method has been developed and validated for the analysis of triptolide in extract from Leigongteng (Tripterygium Wilfordii Hook. F.).The drugs were eluted with petroleum ethyl acetate on a neutral alumina cartridge and then measured by HPLC using a Hypersil C 18 column and acetonitrile water (40∶60, V/V) mobile phase. Triptolide was quantified by ultraviolet absorbance at 210nm. The method proved to be linear in the range of 0.25 4.00mg/L with a regression coefficient of 0.999 7. The lower limit of detection of triptolide was 0.1mg/L. Intra day and inter day coefficients of variation of assay for triptolide were 1.9% 6.5%(n=5)and 2.7% 7.8%(n=5), respectively. The recoveries of the method were 89.8% 92.0%.
出处
《色谱》
CAS
CSCD
北大核心
1998年第4期356-357,共2页
Chinese Journal of Chromatography
关键词
雷公藤甲素
分析
提取物
HPLC
igh performance liquid chromatography, triptolide, pharmaceutical analysis
