摘要
目的 了解植物雌激素大豆苷原 (daidzein)对结肠癌细胞生长的影响。方法 取对数生长期的人结肠癌细胞株LoVo细胞 ,以不同浓度的大豆苷原处理细胞 2 ,3,4和 6d ,然后应用MTT法测定细胞的生长率。结果 大豆苷原在高浓度 10 0 μmol/L时在体外对LoVo有明显的抑制作用 (P <0 .0 1) ,而在低浓度 <5 μmol/L时对LoVo细胞又具有促增殖作用。
OBJECTIVE To study the effect of daidzein, one of the phytoestrogen,on the growth of human colonic cancer cell line LoVo cells. METHOD LoVo cells were cultured in RPMI 1640 cantaining 10% fetal bovine serum and were incubated at 37℃in a humidified atmosphere of 5% CO 2 in air. After 24h, daidzein was added at a varied concentration and detected the growth of LoVo cells by MTT method at 2,3,4 and 6 days.RESULTS A dose dependent inhibition of growth of LoVo cells was observed between 5μmol/L to 100 μmol/L, whereas at the concentration below 5 μmol/L, the daidzein could stimulate the growth of LoVo cells.CONCLUSION Daidzein have a biphasic effect on the growth of LoVo cells.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2003年第3期176-178,共3页
Chinese Journal of Modern Applied Pharmacy
基金
宁波大学人才启动基金 (0 14 -4 710 3 6)
浙江省教育厅科研基金 (2 0 0 10 2 17)
