摘要
目的 :探讨核因子κB(NF κB)在皮质酮损伤海马神经元中的作用。 方法 :一组原代培养的海马神经元直接加入皮质酮 ,于不同的时间提取核蛋白 ,利用凝胶阻滞电泳的方法观察皮质酮对NF κB表达的影响。另一组培养的神经元在加入皮质酮前 2h培养液中加入κBdecoyDNA抑制NF κB表达 ,采用MTT法进一步观察它们对海马神经元的影响。 结果 :NF κB在皮质酮作用下活性表达水平显著下降。NF κB活性被κBdecoyDNA抑制后 ,皮质酮引起的海马神经细胞损伤加重。 结论 :NF κB对皮质酮引起的海马神经元损伤有保护作用。
Objectives: To observe the roles of nuclear factor κB(NF κB) in corticosterone injured hippocampal neurons. Methods:Two groups of primary hippocampal neurons were cultured for 8～10 days. One group was treated with 1×10 -5 mol/L corticosterone. The total cellular nuclear protein levels were extracted at 0.5,1,2 and 4 hours, respectively. Electrophoresis mobility shift assay (EMSA) was used to detect the expression of NF κB activation. Another group was treated with κB decoy DNA(25 μmol/L) to inhibit the activity of NF κB for 2 hours before 1×10 -5 mol/L corticosterone was intervened. MTT was used to observe the effects of corticosterone induced neurons injury after 6,12,24 and 48 hours. Results:The activity of NF κB became significantly lower than that in the control group after corticosterone acted on neurons for 1 hour. As time went on, its effects became more conspicuously. The injury of hippocampal neurons aggravated when the activity of NF κB was inhibited by κB decoy DNA. Conclusions: NF κB plays a protective role in corticosterone induced hippocampal neuron injury.
出处
《医学研究生学报》
CAS
2003年第6期410-412,共3页
Journal of Medical Postgraduates
