摘要
［目的］探讨胃黏膜良恶性病变幽门螺杆菌(Hp)感染与突变型p５３、bcl-２、增殖细胞核抗原（PCNA）表达之间的关系。［方法］用免疫组织化学SP法对１１５例胃黏膜良、恶性病变［慢性浅表性胃炎(CSG)２５例，慢性萎缩性胃炎(CAG)２５例，异型增生(GED)２５例，胃癌(GCA)４０例］的胃镜活检标本进行幽门螺杆菌感染的检测和突变型p５３、bcl-２、PCNA表达的研究。［结果］在CSG、CAG、GED、GCA的各病变中幽门螺杆菌感染检出率分别为３６％(９／２５)、４８％(１２／２５)、７２％(１８／２５)、５８％(２３／４０)；与２０例正常胃黏膜Hp检测阳性率（２０％）作比较，除了CSG无统计学意义外，其余几组均有显著性差异(P＜０．０５)；突变型p５３检出率分别为０、０、２４％(６／２５)、５５％(２２／４０)；胃炎、异型增生、胃癌三者之比有显著性差异(P＜０．０５)；bcl-２检出率４４％（１１／２５）、５２％（１３／２５）、７６％（１９／２５）、５７．５％（２３／４０）；PCNA在CSG、CAG、GED、GCA中的表达呈现递增性，经Ridit检验P＜０．０５；Hp感染与PCNA表达强级(阳性细胞＞５０％)、p５３阳性病例均有显著相关性(P＜０．０１)。［结论］Hp的感染与突变型p５３、bcl-２、PCNA在胃黏膜异型增生。
To investigate the significance of helicobacter pylori(Hp)infection and expression of mutant p53,bcl-2,proliferation cell nuclear antigen(PCNA)in benign and malignant disease of gastric mucosa.The expressions of mutant p53,bcl-2,PCNA and Hp infection were detected by SP immunohistochemical method in25cases with chronic superficial gastritis(CSG),25cases with gastric atrophic gastritis(CAG),25cases with gastric epithelial dysplasia(GED),and40cases with gastric carcinoma(GCA)of gastric mucosa.Hp infection positive rates were36%(9/25)in CSG,48%(12/25)in CAG,72%(18/25)in GED,and58%(23/40)in GCA;mutant p53expression was0,0,24%(6/25),55%(22/40)respectively;bcl-2expression was44%(11/25),52%(13/25),76%(19/25),58%(23/40)respectively.PCNA expression increased progressively in CSG,CAG,GED and GCA.With Ridit analysis there were singnicant difference in the expression between CSG and the other3groups(P<0.05).In GED and GCA the positive rates of p53and PCNA in Hp(+)group were higher than those in Hp(-)group.In GED the positive rates of bcl-2in Hp(+)group were higher than that in Hp(-)group.[Conclusion]The development or genesis of gastric epithelial dysplasia and gastric cancer may be related with the activation of mutant p53,bcl-2and PCNA.p53,PCNA and Hp may be interaction in the carcinogenesis of gastric mucosa.
出处
《肿瘤学杂志》
CAS
2003年第3期135-137,共3页
Journal of Chinese Oncology
