摘要
目的 :研究急性白血病 (AL)中 p5 3、bcl- 2的表达特性及相关性 ,分析其表达与细胞周期的关系。方法 :4 9例 AL 患者为实验组 ,同期住院的骨髓象完全正常的非血液系恶性疾病患者 14例为对照组 ,取骨髓标本 ,分离单个核细胞 ,用特异性单克隆抗体标记 ,流式细胞仪检测突变型 p5 3及bcl- 2基因蛋白表达 ,其中有 2 6例患者同时用流式细胞仪测定了细胞周期。结果 :AL 组 p5 3、bcl- 2蛋白表达阳性率分别为 4 4 .9%、4 9.0 % ,显著高于对照组 ,AL L 组与 ANL L 组差异无显著性 ,M3组显著低于其他非 M3ANL L 组。突变型 p5 3蛋白与 bcl- 2蛋白表达呈正相关 ,二者在白血病的发病上有协同作用。白血病组比对照组细胞周期中 S+G2 / M期细胞数低 ,p5 3、bcl- 2表达与细胞周期无关。结论 :突变型 p5 3、bcl- 2有致白血病的作用 ,二者在白血病的发展中可能起一定协同作用。联合检测突变型 p5 3及 bcl-
Objective:To study the expression of p53?bcl 2 and the relationship among p53?bcl 2 and cell cycle in acute leukemia(AL).Methods:49 patients with AL were selected as a study group and 14 patients without hematologic malignancies in the bone marrow sample were selected as a control group. The patient′s bone marrow was withdrawn and marked with monoclonal antibody. The expression of mutant type p53 protein and bcl 2 protein were detected with flow cytometry.26 patients was detected with flow cytometry for cell cycle at the same time.Results:The expression rate of p53 and bcl 2 proteins in AL group is 44.9 % and 49.0 % respectively,which is significantly higher than those in the control group.There were no difference between ALL group and ANLL group, but was significantly lower in M 3 group than in the other non M 3 ANLL group. Mutant type p53 protein was positively related to the expression of bcl 2 protein, and the two proteins might cooperate in the pathogenesis of leukemia. The numbers of cell in the cycle of S+G 2/M in patients with leukemia were significantly lower than those in control. There were no relationship between cell cycle and the expression of p53?bcl 2.Conclusion:p53 mutation and bcl 2 may be related to leukemia.p53 mutation and bcl 2 expression might cooperate in the progression of leukemia.Combined detection of p53 mutation and bcl 2 expression may be helpful for forcasting the prognosis of leukemia.
出处
《白血病．淋巴瘤》
CAS
2002年第5期282-285,共4页
Journal of Leukemia & Lymphoma
