摘要
目的 观察在高浓度葡萄糖条件下 ,体外培养的兔视网膜 Müller细胞的钙依赖性钾 ( KCa)通道的变化。 方法 高浓度葡萄糖条件下体外培养兔视网膜 Müller细胞 ,并用免疫组织化学染色及透射电镜鉴定培养的 Müller细胞 ,采用膜片钳方法观察不同时间高糖条件下视网膜 Müller细胞 KCa通道的变化。 结果 高糖对体外培养的 Müller细胞 KCa通道有阻滞作用 ,其阻滞作用呈时间依赖性。 结论 高浓度葡萄糖可能通过阻滞 KCa通道而降低 Müller细胞的生物学功能。
Objective To observe the effects of high concentration glucose on the calcium-activated potassium channel of rabbits′ retinal Müller cells. Methods The rabbits′ retinal Müller cells were cultured in vitro under the condition of high concentration glucose, and identified by immunohistochemical staining and transmission electron microscopy. Patch-clamp technique was used to observe the changes of the calcium-activated potassium channel of retinal Müller cells caused by high concentration glucose at different time. Results High concentration glucose could inhibit the calcium-activated potassium channel of cultured retinal Müller cells in a time-dependent manner. Conclusion High concentration glucose may reduce the biological functions of Müller cells by inhibiting calcium-activated potassium channel.
出处
《中华眼底病杂志》
CAS
CSCD
2003年第3期164-167,共4页
Chinese Journal of Ocular Fundus Diseases
基金
吉林省长春市科学基金资助项目 (0 1 - 1 0 6530 )
关键词
高糖
体外培养
视网膜
Mueller细胞膜
离子通道
影响
Retina/drug effects
Glucose/diagnostic use
Endothelial growth factors/analysis
Diabetic retinopathy/physiopathology
Potassium channels
Endothelium, vascular
