摘要
目的 通过研究海人酸致癫痫大鼠海马 GABAB受体亚单位 GBR1a m RNA表达的变化 ,为进一步探明癫痫发病的受体分子生物学机制奠定基础。方法 在立体定位仪下 ,将海人酸注射至大鼠杏仁核制备癫痫模型。将大鼠随机分为正常组和海马致癫痫组 ,分别于不同时间取材 ,进行脑组织 GABAB受体亚单位 GBR1a m RNA原位杂交检测。结果 正常组海马各区均有极少量的 GABAB受体亚单位 GBR1a m RNA的分布 ;海人酸致癫痫组( 6 h,12 h,2 4h) GABAB受体亚单位 GBR1a m RNA水平显著高于对照组 ( P<0 .0 1)。结论 正常大鼠海马各区存在着少量的 GBR1a m RNA表达 ,海人酸致痫后 6 h,12 h,2 4h,GBR1a m RNA的表达水平上调。
Objective To observe the expression of GBR1a mRNA in hippocampus of epilepsy rats.Methods Epilepsy rat models were made by injecting kainic acid into amygdala under stereotactic instrument.50 male Wistar rats were divided randomly into 5 groups:(1)The false operation group;(2～5)Epilepsy group. GBR1a mRNA of brain tissue was tested with the method of in situ hybridization.Results In the normal group,GBR1a mRNA positive cells distributed rarely in hippocampus.GBR1a mRNA positive cells of epilepsy groups were significantly more in hippocampus CA 1,CA 3 and dentate gyrus( P <0.01).Conclusion GBR1a mRNA expresses in rat hippocampus in different degree.It can be up-regulated in epilepsy rats.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2003年第2期110-112,共3页
Journal of Apoplexy and Nervous Diseases
