摘要
目的 了解一氧化氮 (NO)在牙髓神经感觉信息传递中的作用 ,观察牙本质损伤后三叉神经节内和牙髓内还原性辅酶Ⅱ硫辛酸脱氢酶 (NADPH -D)的变化。方法 对大鼠实验性牙本质损伤后牙髓和三叉神经节内 ,以及人牙本质损伤后牙髓内一氧化氮合成酶 (NOS)进行组织化学检测 ,计数统计每个大鼠三叉神经节和牙髓组织标本所有组织切片含NOS阳性细胞总数 ,结果 实验性大鼠牙本质损伤侧三叉神经节内NADPH -D阳性神经数明显多于对照侧 (P <0 .0 5 ) ,而阳性神经元细胞大小未见变化 ;人牙本质损伤牙髓组织内未发现NADHP -D阳性神经元细胞。结论 三叉神经节内的NO可能与牙髓神经痛信息传递和调控有关 。
Objective In order to study the role of Nitric Oxide (NO) in endodontic pain transmission, the changes of NADPH-diaphorase in the neurons of trigerminal ganglion and pulp tissues were studied. Methods The histochemistry of Nitric Oxide Synthase (NOS) in the experimental rat neurons of trigerminal ganglion, dental pulp tissues and human pulp tissues with dentinal stimulation were studied by NADPH-diaphorase (NADPH-D) technique. NOS-positive neurons were counted and statistically analyzed in all collected sections from trigeminal ganglia and pulp tissues. Results NADPH-D positive neurons were scattered in rat trigerminal ganglions. The sizes of positive neurons were not changed. None of NOS-positive fibers was found in human normal and injured pulp tissues.Conclusion The results suggest that NOS in trigeminal ganglion may be qlay an important role in sensory transmission and regulation of pulpalgia. The absence of NOS-positive nerves in human pulp suggests that NO has nothing to do with signal transmission of stimulation in dental pulp tissues.
出处
《临床口腔医学杂志》
2003年第4期198-200,共3页
Journal of Clinical Stomatology
基金
武汉市科委基金 (9861 0 52 2 )
