摘要
目的 :探讨基质金属蛋白酶 - 9在结肠癌中的表达及其与血管生成、侵袭转移之间的关系。方法 :采用免疫组化SP法 ,用MMP - 9抗体标记 4 4例结肠癌组织 ,用第Ⅷ因子抗体标记组织内的血管 ,计数微血管密度 ,用Ⅳ型胶原抗体标记细胞外基质和基底膜。结果 :MMP - 9的表达与性别、肿瘤的位置、分化无相关性(P >0 .0 5 ) ;MMP - 9的表达与淋巴结转移、组织学分型、Dukes分期有明显差异 (P <0 .0 1或 0 .0 5 ) ;结肠癌组织MMP - 9的表达及MVD值均明显高于正常结肠组织 (P <0 .0 5或 0 .0 1) ;结肠癌的MVD值与组织分型、分化、Dukes分期、淋巴结转移均有明显相关性 (P <0 .0 1或 0 .0 5 ) ;MMP - 9表达阳性的结肠癌组织MVD值明显高于表达阴性者 (P <0 .0 1)。结论 :MMP - 9的表达和微血管生成与结肠癌侵袭转移密切相关 ,MMP - 9与结肠癌血管生成密切相关 ,联合检测MMP - 9和MVD对结肠癌的治疗、预后判定有一定的价值。
Objective:To investigate the expression of MMP-9 and intratumor microvessel density in colorectal carcinoma,and their relationship to biological behavior of the cancer?Methods:The MMP-9 expression and MVD of 44 specimens resected from patients with colorectal carcinoma were investigated by immunohistochemical SP method using monoclonal antibody to MMP-9 and Factor Ⅷ and Anti-collagen Ⅳ? Results:There were no correlation between the expression of MMP-9 and sex, carsinoma position, differentiation. The overexpression of MMP-9 was correlated to lymph node metastasis and Dukes grade of carcinoma and tissue type,(P<0.01 and 0.05,respectively )?There were significant different between colorectal carcinoma tissue and normal colorectal tissue in expression of MMP-9 and MVD(P<0.01)?MVD of colorectal carcinoma was significantly correlation with tissue type?differentiation?Dukes grade of carcinoma and lymph node metastasis,(P<0.01 and 0.05,respectively)?The mean MVD in group with MMP-9 positive was significantly higher than that MMP-9 negative (37.11±6.263 VS 23.60±2.408,P<0.01)?Conclusion:The overexpression of MMP-9 and intratumor MVD are strongly related to invasive and metastasis of colorectal carcinoma and may be valuable indictors for selection suitable therapy for patients?
出处
《牡丹江医学院学报》
2003年第2期1-5,共5页
Journal of Mudanjiang Medical University
基金
黑龙江省自然科学基金资助项目D0 0 -63
