摘要
采用圆二色谱和荧光光谱 ,研究了黄精凝集素Ⅱ (PCLⅡ )在不同温度及不同pH值时 ,其活性和分子构象变化的关系 ,结果表明 :PCLⅡ具有较强的温度和酸碱度的耐受性 ,但在80℃ ,pH 2 ,pH 4和 pH 12时 ,其分子构象发生较大变化 。
Circular dichroism(CD) and fluorescence spectra were used to elucidate the relationship between the activity and molecular conformation of Polygonatum cyrtonema Hua.,Lectin Ⅱ(PCLⅡ) under different temperature and pH Value. The results showed that PCLⅡ was stable in various temperature and pH between pH 4～9.5 and below 80℃, but the molecular structure and biological activity changed much under pH 2,pH 4,pH 12 and 80℃, and the activity of PCLⅡ became weak.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第2期371-376,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金 (3 9770 178)
