摘要
目的 :探讨人肝癌中热休克蛋白 70 (HSP70 )与 p5 3的相互作用。方法 :用免疫组织化学染色法 ,从 12例肝癌组织中筛选HSP70与 p5 3均呈阳性表达的标本 ,并以免疫共沉淀法提取之。然后用SDS PAGE及Westernblot分析双阳性标本中两种蛋白的存在形式。结果 :用免疫组织化学法检测到 12例肝癌组织中有 3例为双阳性 ,用抗HSP70mAb免疫共沉淀的样品 ,可检测到 p5 3蛋白。用抗p5 3mAb免疫共沉淀的样品也可检测到HSP70蛋白。结论 :人肝癌中p5 3与HSP70以复合物的形式而存在 。
AIM: To investigate the interaction between p53 and heat shock protein70 (HSP70) in human hepatocellular carcinoma tissues. METHODS: HSP70 and p53 double positive samples were screened by imunohistochemical staining from twelve human hepatocellular carcinoma tissues, and the tissue homogenate was prepared. Coimmunopitation was performed by using either anti HSP70 mAb or anti p53 mAb. The immunoprecipitate was analysed by SDS PAGE and Western blot. RESULTS: Immunohistochemical staining detection showed that three samples out of twelve cases were double positive for both HSP70 and p53. p53 protein existed in the immunoprecipitate of anti HSP70 mAb, while there was HSP70 protein in the immunoprecipitate of anti p53 mAb. CONCLUSION: HSP70 and p53 exist as a complex in human hepatocellular carcinoma tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human hepatocellular carcinoma.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第2期195-196,199,共3页
Chinese Journal of Cellular and Molecular Immunology
