摘要
目的:探讨ERK1/2和Survivin在食管癌中的表达和二者之间的相关性。方法:应用免疫组化法检测40例食管癌和20例正常食管标本中ERK1/2和Survivin的表达。Westernblot法检测ERK1/2和Survivin在食管癌中的表达以及PD98059对食管癌Eca109细胞株ERK1/2、Survivin表达的影响。免疫共沉淀法检测ERK1/2和Survivin在食管癌Eca109细胞株中的相互作用。结果:ERK1/2和Survivin在食管癌中表达的阳性率分别87.5%和90.0%,在正常食管组织中表达的阳性率为40.0%和5.0%。Westen blot显示ERK1/2和Survivin在食管癌癌组织中的表达高于癌旁组织中的表达。免疫共沉淀结果提示ERK1/2和Survivin在食管癌中的表达存在相互作用。PD98059下调Survivin的表达。结论:ERK1/2和Survivin在食管癌中高表达,在食管癌Eca109细胞中ERK1/2和Survivin表达有相关性,抑制ERK1/2的表达能下调Survivin的表达。在食管癌中Survivin的表达可能依赖于ERK1/2。
Objective：To investigate the expression and correlation of ERK1/2 and Survivin in esophageal cancer. Methods：The expression of ERK1/2 and Survivin on 40 esophageal cancer tissues and 20 normal esophageal tissues were detected by immunohis- toehemistry. The protein expression of ERK1/2 and Survivin and the consequent changes by PD98059 were detected by Western blot analysis. The correlation of ERK1/2 and Survivin in Ecal09 cells was detected by co-immunopreeipitation analysis. Results： Among 40 esophageal cancer tissues,the positive immunolabelling rate of ERK1/2 and Survivin was 87.5% and 90.0% respectively, while among normal esophageal tissues the positive rate was 40.0% and 5.0%. The protein expression levels of ERK1/2 and Survivin in esophageal cancer tissues were higher than those in paraneoplastic tissues. There was coexpression of ERK1/2 and Survivin proteins. PD98059 down-regulated the expression of Survivin in Ecal09 cells. Conclusion：The expression of ERKI/2 and Survivin is remarkably up-regulated in esophageal cancer tissues than in paraneoplastic tissues. There is positive correlation between the expression of ERK1/2 and Survivin in Ecal09 cells. Inhibition of ERK1/2 by PD98059 induces down-regulation expression of Survivin. The expression of Survivin on esophageal cancers may depend on ERK1/2 expression.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第8期1085-1088,1103,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省科技厅基础研究计划资助项目(BK2005153)
