摘要
探讨用流式细胞术检测免疫表型对诊断阵发性睡眠性血红蛋白尿(paroxysmalnoctur-nalhemoglobinuria,PNH)的意义。方法:用荧光标记分化群(clusterofdifferentiation,CD)中的CD55、CD59的单克隆抗体,采用流式细胞术测定25名正常人、28例PNH患者外周中性粒细胞和红细胞膜抗原的表达。结果:PNH患者中性粒细胞CD55、CD59,红细胞CD59表达率较正常人明显降低,且与疾病的严重程度相关。结论:用流式细胞术检测CD55、CD59表型是目前诊断PNH的较直接而又特异、敏感的方法。
Objective:To evaluate the significance of detection of CD55,CD59 phenotype in the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). Methods:Expressions of CD55 and CD59 on neutrophil and erythrocyte were analyzed with flow cytometry in 25 normal subjects and 28 cases of PNH patients. Labeled anti CD55 and CD59 monoclonal antibodies by fluorescence were used. Results:CD55 and CD59 expressions on neutrophil and CD59 in erythrocyte were significantly decreased in PNH patients,which was related to the severity of the disease. Conclusion:Determination of CD55 and CD59 in erythrocyte and neutrophil can be considered as a direct specific and reliable method for the diagnosis of PNH.
出处
《新医学》
北大核心
2003年第4期212-213,共2页
Journal of New Medicine
关键词
流式细胞术
检测
CD55
CD59
表型
诊断
阵发性睡眠性血红蛋白尿
Immunophenotype Paroxysmal nocturnal hemoglobinuria Cluster of differentiation 55Cluster of differentiation 59 Flow cytometry
