摘要
为了探讨两种具有协同作用的细胞因子共转染的瘤苗是否具有更好的抗肿瘤作用，我们将ＴＮＦ－α基因和ＩＬ－２基因转染Ｂ１６黑色素瘤细胞，获得同时分泌ＩＬ－２和ＴＮＦ的Ｂ１６细胞克隆株（命名为Ｂ１６－ＩＬ－２＋－ＴＮＦ－α＋细胞），并观察了其体内致瘤性和瘤苗效果。结果发现，Ｂ１６－ＩＬ－２＋－ＴＮＦ－α＋细胞的体内致瘤性显著低于ＴＮＦ－α基因或ＩＬ－２基因单独转染的Ｂ１６细胞；而且，事先接种Ｂ１６－ＩＬ－２＋－ＴＮＦ－α＋细胞的小鼠能抵抗再接种的野生型肿瘤细胞的生长，表明其具有更佳的瘤苗效果。
TNF α gene and IL 2 gene were co transfected into B16 melanoma cells.The B16 cell clone secreting IL 2 and TNF α(named B16 IL 2 + TNF α + cell)was obtained,and its in vivo tumorigenicity and vaccine effect were observed.Found that the tumorigenicity of the TNF α gene and IL 2 gene co transfected B16 cells decreased significantly as compared with either IL 2 gene or TNF α gene transfected B16 cells.In addition,the B16 IL 2 + TNF α + cellimmunized mice could inhibit the growth of the rechallenged wild type B16 cells,suggesting that IL 2 gene and TNF gene cotransfected tumor cells could be used as tumor vaccine mith higher efficacy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1997年第2期77-80,共4页
Chinese Journal of Immunology
基金
国家自然科学基金
