摘要
目的 探讨新型基因工程人肿瘤坏死因子（ｎｒｈ Ｔ Ｎ Ｆ） 和足叶乙甙（ Ｖ Ｐ－ １６） 的协同抗肿瘤作用及ｎｒｈ Ｔ Ｎ Ｆ 的毒副作用。方法 以复制成功的 Ｌｅｗｉｓ 肺癌（３ Ｌ Ｌ） 小鼠为模型局部用药。结果 ｎｒｈ Ｔ Ｎ Ｆ 或 Ｖ Ｐ－ １６ 瘤体内注射均能使肿瘤生长及肺转移受到一定的抑制，抑瘤率分别为３３７１ ％ 、３０４６ ％ ，肺转移瘤数与对照组比较 Ｐ ＜ ００５ ，肿瘤出现一定程度坏死。而联合用药则能显著抑制肿瘤生长及肺转移，抑瘤率为６５７７ ％ ，大于理论抑瘤率５３９１ ％ ，肺转移瘤数与对照组比较 Ｐ ＜ ００１ ，肿瘤出现广泛的出血坏死。同时观察到ｎｒｈ Ｔ Ｎ Ｆ 对实验小鼠无明显毒副作用。结论 ｎｒｈ Ｔ Ｎ Ｆ 和 Ｖ Ｐ－ １６联合应用，具有协同抗肿瘤作用，对于肺癌治疗具有进一步临床应用探讨价值。目的 探讨新型基因工程人肿瘤坏死因子（ｎｒｈ Ｔ Ｎ Ｆ） 和足叶乙甙（ Ｖ Ｐ－ １６） 的协同抗肿瘤作用及ｎｒｈ Ｔ Ｎ Ｆ 的毒副作用。方法 以复制成功的 Ｌｅｗｉｓ 肺癌（３ Ｌ Ｌ） 小鼠为模型局部用药。结果 ｎｒｈ Ｔ Ｎ Ｆ 或 Ｖ Ｐ－ １６ 瘤体内注射均能使肿瘤生长及肺转移受到一定的抑制，抑瘤率分别为３３７１ ％ 。
Objective To investigate the effect of new recombinant human tumor necrosisfactor(nrh T N F) combined with etoposide( V P- 16) on inhibition of murine lung cancer and the side effect of T N Fin experimental mice . Methods Tumor- bearing mice wererandomly divided into four groups ,normalsaline ,nrh T N F, V P- 16 and nrh T N Fplus V P- 16 were injected into the neoplasm ofthe fourgroups rospectively . Four mice of each group were killed 14 days afterinoculation after,neoplasms were detected underlight microscope ;others were killed after 21 days inoculation ,neoplasms were weighted ,and lung metastatic tumors were counted . Results Both nrh T N Fand V P- 16 inhibited the growth and lung metastasis of murine 3 L Ltumor,inhibition ratesin nrh T N Fgroup and V P- 16 group were 33 .71 % and 30 .46 % ,respectively ,and tumor necrosis resulted to a certain degree . The lung metastatic tumors in the groups mentionedabove were fewer than in control group ( P < 0 05) . Afavourable out- come was observed in the animals treated with nrh T N F/ V P-16 ,i.e .lower tumor growth rate (inhibition rate 65 .77 % ) and more extensivetumor hemonagic necrosis oppeareathan in control group( P < 0 01) .nrh T N Fprodtced no marked side ef
出处
《武警医学》
CAS
1999年第9期497-499,共3页
Medical Journal of the Chinese People's Armed Police Force
关键词
人肿瘤坏死因子
足叶乙甙
肺癌
治疗
Recombinant human tumor necrosisfactor Etoposide Lung cancer
