摘要
目的 对 4种不同的梅毒螺旋体抗体检测方法进行比较 ,筛选高敏感性和特异性诊断方法和试剂。方法 采用快速血浆反应素试验 (RPR) ;梅毒螺旋体特异性抗体明胶凝集试验 (TPPA) ;梅毒螺旋体特异性抗体酶联免疫吸附试验 (TP- EL ISA) ;梅毒螺旋体特异性抗体乳胶凝集试验 (TPL A) 4种方法对 76份可疑梅毒抗体阳性标本进行检测。结果 RPR有一定假阳性和假阴性 ;TPPA敏感性稍差 ;TP- EL ISA敏感性最高。结论 TP- EL ISA敏感性高 ,适合大批献血员筛查 ;TPL A与其他方法的检测结果符合率最高 ,且操作简便 。
OBJECTIVE To compare the four Treponema pallidum antibody (TP AB) detecting tests with 76 TP AB presumptive positive serum samples to evaluate which one has higher sensitivity and specificity. METHODS Rapid plasma reagent (RPR); passive particle agglutination test for detection of TP AB (TPPA); Treponema pallidum enzyme linked immunosorbent assay (TP ELISA); Treponema pallidum latex assay (TPLA)were used. RESULTS RPR had certain false positive and false negative results and has poorer sensitivity; TP ELISA showed the highest sensitivity; TPLA gave the best correlation with the other methods. CONCLUSIONS TPLA is more suitable for TP confirmatory test in clinic labs.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第1期87-88,共2页
Chinese Journal of Nosocomiology
