摘要
目的 探讨血小板型磷脂酶 A2 在宿主防御细菌感染中的作用 ,使我们对 PL A2 分子有新的认识。方法 大鼠静脉注射活金黄色葡萄球菌造成细菌感染模型 ,分别抽取不同时间段的静脉血 ,进行血浆杀菌实验、PL A2酶活性及全血 PL A2 m RNA含量测定。结果 经过活菌刺激后 ,3 h大鼠血浆中 PL A2 的活性升高了 2 7倍 ,此时血浆对革兰阳性细菌具有较强杀灭作用 ;全血中 PL A2 m RNA含量也有所增加 ,1h达最高值。结论 血小板型PL A2 在宿主防御
OBJECTIVE To explore the role of rat platelet phospholipase A 2 (PLA 2) in host defense against bacterial infection and to further understand its function. METHODS The bactericidal activities, PLA 2 activity of plasma and PLA 2 mRNA content of blood collected serially from rat before and after intravenous injection of Staphylococcus aureus were measured. RESULTS After injection of bacteria, the PLA 2 activity of plasma collected at 3h rose 27 folds, and possessed potent bactericidal activity towards Gram positive bacteria, and the mRNA in blood also rose to peak at 1 h. CONCLUSIONS Rat platelet PLA 2 play an important role in host defense against bacterial infection.
出处
《中华医院感染学杂志》
CAS
CSCD
2003年第1期10-12,共3页
Chinese Journal of Nosocomiology
基金
广西自然科学基金资助项目 (992 0 0 12)
国家人事部留学回国人员科技资助项目
