摘要
目的 测定肾替代治疗的烧伤患者肾脏透析排出液中头孢噻肟钠的含量。方法 用HPLC ,μBondapak C18柱 ,流动相为甲醇 -醋酸钠 (0 0 2mol·L-1,pH 5 0 ) (30∶70 ) ,流速 1 2ml·min-1,检测波长 2 6 0nm。结果 在 1～ 10 0 μg·ml-1的范围内线性良好 ,r =0 9999;日内、日间RSD≤ 2 % ,平均回收率 10 0 2 %。结论 所用方法简便、快速、准确、可用于样品测定。
OBJECTIVE To determine Sodium cefotaxime in renal dialysate from burn injured patients .METHODS HPLC was used.The sample was separated on μ Bondapak C 18 analysis column.The mobile phase was CH 3OH-NaAc(0.02 mol·L -1 pH 5 0)(30∶70),and flow rate was 1.2 ml·min -1 .Detective wavelength was 260 nm.RESULTS A linearity was obtained from 1-100 μg·ml -1 with a good correlation (r=0.9999).RSD of within day and between days were less than 2%.The average recovery was 100.2%.CONCLUSION The method is simple,rapid and accurate.
出处
《华西药学杂志》
CAS
CSCD
2003年第1期60-61,共2页
West China Journal of Pharmaceutical Sciences
