摘要
[目的]建立反相高效液相色谱法测定大鼠胰腺组织中头孢噻肟钠的含量。[方法]以茶碱为内标,采用YMC-C18柱,以0.015mol/L磷酸二氢钾缓冲液-甲醇溶液(pH调4.70)(82︰18)为流动相,流速0.8ml/min,紫外检测波长为254nm。[结果]标准曲线线性范围为:16～0.5(μg/ml);头孢噻肟的检测限为0.25μg/ml;提取回收率为:76.70%～85.24%;方法回收率为106.21%～107.79%;日内RSD在5.74%～10.76%之间,日间RSD在5.53%～9.14%之间。[结论]该方法操作简单,结果准确,重现性好,用于大鼠胰腺组织中的头孢噻肟钠的含量测定得到满意结果。
[Objective] To develop a RP-HPLC method then determine the concentration of Cefotaxime in rat＇s pancreas. [Methods] Chromatographic separation was performed on a YMC C18 column （150mm ×4.6 mm, 5μm） eluted with a mobile phase composed of potassium di-hydrogen phosphate buffer （0.015 mol/L; pH=4.70） -methanol （82/18, V/V） with a flow rate of 0.8ml/min. The detection wavelength was set at 254 nm. Theophylline was used as an internal standard. [Results] The method was validated over a range of 0.25-16μgg/ml with correlation coefficient of 0.999. Other quantitative outcomes showed as the limit of quantitation for the Cefotaxime was 0.25 μg/ml; the extraction recovery for the Cefotaxime averaged from 76.70% to 85.24%; the methodological recovery for Cefotaxime ranged from 106.21% to 107.79 %; inter-day RSD of 5.74%-10.76% and intra-day RSD of 5.53%-9.14%, respectively. [Conclusion] The sensitive, accurate and easily manipulated assay offers a satisfactory tool in the investigation of pharmaeokinetics and relative bioavailability of Cefotaxime.
出处
《现代预防医学》
CAS
北大核心
2008年第19期3766-3767,3771,共3页
Modern Preventive Medicine
基金
四川省科技厅重点攻关项目(05SG011-021-1)
