摘要
目的 探讨三七皂甙单体Rg1 、Rb1 (三七Rg1 、Rb1 )在脑损伤时对脑细胞凋亡和分泌型磷脂酶A2 (secretoryphospholipaseA2 ,sPLA2 )及其相关介质的影响。方法 用线栓法制成大脑中动脉缺血再灌注 (middlecerebralarteryischemia reperfusion ,MCA IR)模型 ,用原位末端标记 (TUNEL)法检测脑细胞凋亡 ,用免疫组织化学法检测sPLA2 在脑细胞中的表达 ,用3H标记大肠杆菌膜为底物的液闪方法和酶联免疫吸附测定 (ELISA)法 ,分别检测血清中sPLA2 活性和肿瘤坏死因子 α(TNF α)、前列腺素E2 (PGE2 )水平。结果 在大鼠MCA IR脑损伤时 ,三七Rg1 、Rb1 明显降低凋亡细胞数和血清中sPLA2 、TNF α、PGE2 水平及sPLA2 在脑细胞中的表达 ,与对照组相比 ,差异有显著性意义 (P <0 .0 1)。结论 三七Rg1 、Rb1 对MCA IR脑损伤有良好的保护作用 ,其机制在于抑制sPLA2 的激活、表达及其相关介质的水平。
Objective To study the mechanism of actions of Rg 1 and Rb 1 on cerebral apoptosis, secretory phospholipase A 2 (sPLA 2) and related mediators.Methods Thread technique was used to establish ischemic model of middle cerebral artery ischemia reperfusion (MCA IR).Apoptosis was measured by TUNEL staining. sPLA 2 activity was examined by means of E.coli membrane labelled by oleic acid. The levels of TNF α and PGE 2 were measured by ELISA test. sPLA 2 protein expression was measured by immuno histochemistry.Results Panax Notoginseng saponins Rg 1 and Rb 1 lowered the levels of sPLA 2,TNF α and PGE 2 in MCA IR cerebral injury of rats remarkably and also inhibited sPLA 2 protein expression as compared with the control group,with significant statistical diffence(P< 0.01 ).Conclusions Panax Notoginseng saponins Rg 1 and Rb 1 have good protective effect on MCA IR injury of rats. The mechanism of anti cerebral injury action of Rg 1 and Rb 1 is considered to lower levels of sPLA 2,TNF α and PGE 2 and to inhibit the expression of sPLA 2 protein.
出处
《中华老年心脑血管病杂志》
CAS
2003年第1期57-59,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
